Plant Disease Research

  • Year: 2014
  • Volume: 29
  • Issue: 2

First record of Capsicum chlorosis virus infecting capsicum in Himachal Pradesh

  • Author:
  • Ajay Brakta, Yogita Bohra, Anil Handa, P.D. Thakur
  • Total Page Count: 4
  • DOI:
  • Page Number: 193 to 196

Department of Plant Pathology, College of Horticulture, Dr. Y.S. Parmar University of Horticulture & Forestry, Nauni (Solan)-173230

Abstract

The present study confirms the presence of Capsicum chlorosis virus (CaCV), a member of genus Tospovirus in Bell Pepper (Capsicum annuum L.) from himachal Pradesh based on serology and RT-PCR. Typical ringspot symptoms were observed on leaves and fruits under glasshouse conditions as well as in open fields. Further, molecular identification of the virus isolate was done by cloning and sequencing of the test isolate. Sequence analysis of the complete nucleocapsid gene showed 99 per cent nucleotide similarity in BLASTn analysis with CaCV isolate from tomato (accession no. EU216023) and phylogenetic studies revealed that the isolate under study shared common ancestry with CaCV isolate from Amaranthus species (HG917416).

Keywords

Capsicum, CaCV, Ringspots, ELISA, RT-PCR