Plant Disease Research

  • Year: 2015
  • Volume: 30
  • Issue: 2

Isolation and molecular characterization of rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae from Andaman islands, India

  • Author:
  • K. Sakthivel1, R. K. Gautam1,, K. Manigundan1, P. K. Singh1, G.S. Laha2, R. Velazhahan3
  • Total Page Count: 6
  • DOI:
  • Page Number: 136 to 141

1Division of Field Crop Improvement and Protection, ICAR-Central Island Agricultural Research Institute, Port Blair-744 101

2Division of Plant Pathology, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad-500 030

3Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore-641 003

Abstract

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious problem to rice cultivation in Andaman islands. A total of 24 Xoo isolates were collected from different rice growing areas of Andaman Islands. All isolates were identified through a series of morphological, biochemical and molecular approaches. Molecular variability among the isolates was analysed through PCr using multi-locus Jel1 and Jel2 primers which targeted repetitive IS1112 fragment of the pathogen. The results showed the grouping of isolates into 2 major clusters with high genetic variability among the isolates. Cluster i comprised 15 and cluster ii comprised 9 isolates. no correlation between genetic diversity in the pathogen and geographical origin of the isolates was however observed. The information generated in the present study would be much helpful to have a basic idea on genetic nature of pathogen in Andaman islands and to formulate successful BB disease management strategies with respect to island conditions.

Keywords

Andaman islands, bacterial blight, genetic diversity, rice