1Department of Plant Pathology, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004
2School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004
3Department of Agricultural Botany, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt
*E-mail: narindermutti@pau.edu
Online published on 21 April, 2018.
Seventeen isolates of Pseudomonas fluorescens were isolated from the rhizospheric soil samples collected from rice fields. The antagonistic activity of these isolates against Rhizoctonia solani and Rhizoctonia oryzae causing sheath blight of rice in dual culture revealed that the P. fluorescens (Pf14) isolate reduced the mycelial growth of R. solani by 60.49% (inhibition zone 9.6mm) and for R. oryzae Pf14 followed by Pf11 exhibited the maximum inhibition with the highest inhibition zones of 12.78 and 11.56 mm, respectively. Volatile and non-volatile compounds of Pf14 caused the maximum inhibition of R. solani (95.31% and 76.79%) and R. oryzae (93.46% and 66.79%). highest chitinase and β-1, 3-gulucanase production was recorded in Pf14 i.e. 17.5 unit/ml and 1.76 unit/ml, respectively. Pf14 isolate produced highest value of siderophores as compare to other isolates. Application of Pf14 under greenhouse conditions reduced the disease severity upto 81.03 per cent by seedling root dip + foliar spray treatment.
Pseudomonas fluorescens, Rhizoctonia solani, Rhizoctonia oryzae, chitinase, siderophores
