Shelf life study of the talc based bioformulation of the Trichoderma asperellum and Pseudomonas fluorescens, bioagents of plant pathogens

The talc based bioformulation of Trichoderma asperellum and Pseudomonas fluorescens were evaluated for their shelf life at different temperatures (ambient, 25°C, 4°C and 0°C). The initial microbial count for the fungal antagonists (T. asperellum) was 3.3×10⁸ Cfu/g at zero day which was reduced to 6.9×10³ after 210 days at ambient temperature and to 2.9×10⁴ after 365 days at zero temperature. For the bacterial antagonist i.e. P. fluorescens the initial inoculum density at zero day was 2.6×10⁸ which gradually decreased to 3.7×10⁵ after 210 days at ambient temperature and to 3.3×10⁴ after 365 days at 0°C.