Molecular characterization of Okra yellow vein mosaic virus infecting okra in south Gujarat

The present study was conducted to characterize a Begomovirus infecting okra in south Gujarat region showing typical yellow vein mosaic disease symptom. Part of DnA-A molecule of ∼1200bp was amplified with a Begomovirus specificprimers. On sequencing, a 1140 bp nucleotide sequence was obtained. Amplified fragment had two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene. The sequenced virus showed highest identity (98%) with Okra yellow vein mosaic virus (Aurangabad) segment DnA-A (GU181356.1). Accordingly the sequenced isolate has been considered as tentative strain of Okra yellow vein mosaic virus [Aurangabad] and named as Okra yellow vein mosaic virus [Aurangabad] [India: nvs: Okra: 2016] and was abbreviated as OYVMV [Aurangabad] [India: nvs: Okra: 2016]. Study of the phylogenetic tree of the viruses from the different crops and geographical area revealed that the virus isolated and sequenced during the present investigation was closely related to the viruses of okra and cotton from the different parts of the world.