Plant Disease Research

Nine Trichoderma isolates collected from rhizospheric soil of Medziphema were evaluated in terms of mycelia growth rate, colony characters and sporulation in five different growth media. The highest growth was found in modified PDA(48.02 mm/day) followed by PDA(42.82) and least growth in CAM (36.78). All the growth media were utilized by TrichoFL-1 (46.88 mm/day) followed by TrichoFL-5 (45.81), TrichoBG-3 (45.72), & TrichoR-2 (45.62) whereas TrichoR-8 (35.87) and TrichoR-7 (37.71) showed least growth rate. Variations in colony colour, texture, margin and hyphal thickness were observed on each culture medium for each Trichoderma isolate. Compact and moderately compact texture was found in PDA and WAM while loose and woolly colony texture was observed on MPDA and CAM for almost all the isolates. The sporulation was highest on WAM (14.09 ×10⁷ spores/ml), followed by PDA (13.23×10⁷), MEA medium (7.72×10⁷), CAM (5.57×10⁷) and least number of spores were obtained on MPDA (3.21×10⁷) for all the isolates. Among the Trichoderma isolates, maximum spore production capacity was in the case of TrichoFL-5 (13.44×10⁷) followed by TrichoS-9 (11.57×10⁷) and TrichoFL-1 (10.10×10⁷) and the lowest capacity was in case of TrichoR-7 (5.11×10⁷) followed by TrichoC-4 (6.11×10⁷) and the remaining isolates.