Effect of length, season of collection of explant and nutrient media on In Vitro shoot sprouting and proliferation rate of R. Chinensis var. Minima Cv. Don Don
Abstract
Effect of different explant sizes and seasons of explant collection on in vitro explant sprouting and BA (benzyl adenine) on shoot proliferation were studied in the miniature rose cultivar ‘Don Don’. The explants of 0.5–1.5 cm length were collected in different seasons and were cultured on standardized media. The sprouted shoots were transferred in MS media supplemented with different levels of BA and IBA for shoot proliferation. In general, the maximum per cent sprouting was obtained by explants with greatest length (1.5 cm) collected in spring season after 21 days of inoculation and maximum shoot proliferation was observed in MS media supplemented with 2.0 mgl−1 BA after second subculturing.
