Protocol standardization for micropropagation of Citrus jambhiri Lush. using nodal segments of nucellar seedlings

The present study was conducted during 2008–09 to standardized micropropagation protocol for rough lemon (Citrus jambhiri Lush.: Rutaceae) using nodal segment of nucellar seedlings. The maximum survival of explants (100 per cent) and minimum time required to bud breaking (17.00 days) were observed with BAP 1.0 mgl⁻¹ + kinetin 1.0 mgl⁻¹, and BAP 0.5 mgl⁻¹ + kinetin 1.0 mgl⁻¹, respectively whereas maximum number of shoot (8.30) and length (2.60 cm) were recorded with BAP 2.0 mgl⁻¹ + kinetin 1.0 mgl⁻¹ and BAP 1.0 mgl⁻¹ + kinetin 1.0 mgl⁻¹ respectively. The plain MS medium unable to initiate rooting process but slight adding of hormone either NAA or IBA enhancesd the process. The maximum (90 per cent) survival of microshoot for rooting on NAA (0.5 mgl⁻¹)+ IBA (0.5 mgl⁻¹), minimum time taken to root induction (19.30 days) on NAA (0.1mgl⁻¹)+ IBA (1.0 mgl⁻¹) maximum number of root (5.90) on NAA (0.1 mgl⁻¹)+ IBA (0.5 mgl⁻¹) and maximum length of root (6.60cm) on NAA (0.5 mgl⁻¹)+ IBA (0.5 mgl⁻¹) were recorded. The rooted plantlets were successfully acclimatized in green house, in pot containing sterile soil, perlite and vermiculite in equal proportion and found 85.00 per cent success after 60 days of transfer in glass house.