Micropropagation of strawberry plants through nodal culture

The present experiment was conducted to develop an efficient in vitro protocol for rapid multiplication of strawberry cv. Camarosa. Nodal segments of strawberry were cultured in MS medium supplemented with different concentrations of NAA with BAP. The best response of shoot multiplication was obtained in MS medium containing 0.2 mg l^-1 NAA + 1.5 mg l^-1 BAP. However, the shoot length, shoot weight, number of leaves and leaf length was recorded the best in MS media fortified with 0.2 mg l^-1 NAA + 2.5 mg l^-1 BAP. Following shoot development, the micro-shoots were transferred to root induction media, where, MS media containing 1.0 mg l^-1 IBA was found to be efficient for the maximum root induction capacity, minimum days to roots in shoots, maximum number of roots, longest roots, maximum weight of the developed roots. The plantlets, thus developed, were successfully acclimatized in a mixture of coco peat and vermiculite (1:1).