Manipulation of culture requirements for low cost in vitro Tuberization in Potato

Media and culture conditions have been standardized for efficient shoot proliferation and microtuber induction for potato cultivars Kufri Bahar, Kufri Badshah and Kufri Lalima. For economy of the in vitro micropropagation process, expensive ingredients like analytical grade chemicals and sucrose were replaced with commercial grade chemicals and table sugar. Distilled water was substituted with tap water. It has been demonstrated that the cost reduction resulting from these substitutions is not accompanied by any adverse impact on the efficiency of micropropagation. The procedure offers an economically viable alternative to field production of seed potatoes.