Potato Journal
  • Year: 2003
  • Volume: 30
  • Issue: 1 and 2

Cloning and sequencing of an Osmotin-like gene fragment from the late blight resistant wild potato species Solanum chacoense

  • Author:
  • Jeena Banyal, Meetul Kumar, SK Chakrabarti, D Pattanayak, VP Chimote1, Lokendra Singh2, PS Naik1
  • Total Page Count: 2
  • Page Number: 37 to 38

1Division of Crop Improvement, Central Potato Research Institute, Shimla-171 001, HP.

2Department of Botany, Meerut College, Ch. Charan Singh University, Meerut, UP.

Abstract

An osmotin-like gene has been cloned and sequenced from the late blight resistant wild potato species Solanum chacoense. Expression of the cloned gene in Escherichia coli resulted in a new protein of expected size whose toxicity against Phytophthora infestans is being evaluated in vitro. The gene has also been sub-cloned in a binary vector for future use in Agrobacterium-mediated genetic transformation of potato.