A field experiment was conducted to study the accumulation of dry matter and nutrients in different parts of the potato (Solanum tuberosum L. var. Kufri Jyoti) under different levels of soil fertility on acidic soil of Simla. There was a positive response of the potato to N, P and K. Their combined application almost doubled dry matter yield and the total contents of nutrients (N, P, K, S, Ca and Mg) in the plant due to higher rates of absorption. The concentration of the nutrients in different plant parts was, however, not affected by the application of N, P and K. The concentration of nutrients was higher in the tops than the tubers. Tubers continued to accumulate nutrients upto maturity whereas the tops started to deplete invariably after 65 to 75 days of planting. Tuberisation and early bulking stage (65 to 85 days) were found to be critical for the potato, though adequate nutrient supply was essential throughout the growing season. The translocaton of photosynthates and nutrients from the tops to the tubers was also accelerated by the fertilizers. The potato crop, supplied with adequate N, P and K removed 117.5 kg N, 14.3 kg P, 193.1 kg K, 13.4 kg S, 37.1 kg Ca and 63.1 kg Mg pet hectare. Tubers accounted for more than 70 percent of tbe nutrient removal by plant except Ca whose removal by tubers was 23 percent.