1Division of Plant Pathology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560 089, India
2Department of Post-Graduate Studies and Research in Biotechnology, Bioscience Block, Kuvempu University, Jnanasahyadri, Shankaraghatta-577 451, India
*E-mail address: pallem@iihr.ernet.in
Online published on 29 August, 2013.
Chitinases are an effective tool for the complete degradation of mycelia or conidial walls of phytopathogenic fungi. A gene encoding chitinase was cloned from Trichoderma atroviride to test its activity against fungal pathogens. Genomic DNA was isolated from the Trichoderma atroviride isolates, OTPT2 and OTPT3. Chitinase genes of lkb were isolated using chitinase specific primers. The genes were cloned into pTZ57R/T vector and transformed into E.coli DH5a. The genes were sequenced and found to have high homology with the other reported Trichoderma chitinase sequences (GenBank AY028421, AY129675). The genes were cloned into pET27b protein expression vector and transformed into BL21 cells. The predicted protein contains 340 amino acids. Chitinase protein was expressed and isolated.
Chitinase, Trichoderma atroviride, cloning
