Pesticide Research Journal
SCOPUSWeb of Science
  • Year: 2016
  • Volume: 28
  • Issue: 2

Metabolic Mechanisms of Insecticide Resistance in Spodoptera litura (Fab) on Groundnut

  • Author:
  • E Chandrayudu, T Murali Krishna, M John Sudheer, P Sudhakar, K Vemana
  • Total Page Count: 9
  • Page Number: 201 to 209

Department of Entomology, S.V. Agricultural College, Acharya N.G. Ranga Agricultural University (ANGRAU), Tirupati, 517 502, Andhra Pradesh, India

*Corresponding author E-mail: ecrayudu@gmail.com

Online published on 21 March, 2017.

Abstract

Activity of acetylcholinesterase, carboxyl esterase and glutathione-S-transferase enzymes is reported in resistant and susceptible third instar larvae of Spodoptera litura. Field collected strains of S. litura from Guntur, Karimnagar and Kurnool recorded significantly high acetyl cholinesterase (0.45 ± 0.04 to 0.98 ± 0.20, 0.13 ± 0.11 to 0.90 ±0.23 and 0.15 ± 0.11 to 0.90 ± 0.18 μmoles min−1 mL−1), carboxyl esterase (128.60 ± 0.32 to 532.29 ± 0.20, 95.79 ± 0.32 to 723.49 ± 0.21 and 77.16 ± 0.19 to 558.24 ± 0.22 nmoles min−1 mL−1) and glutathione-S-transferase (92.41 ± 1.07 to 124.58 ± 1.14, 31.44 ± 0.92 to 79.37 ± 1.02 and 28.38 ± 1.06 to 101.2 ± 1.16 μmoles min−1 mL1) activities which were followed by Chittoor, Mahaboobnagar and Nellore strains respectively as compared to the base line susceptible population (0.04 ± 0.01 to 0.23 ± 0.03 μmoles min−1 mL−1, 94.31 ± 0.12 to 378.08 ± 0.18 nmoles min−1 mL−1 and 21.25 ± 0.89 to 69.44 ± 1.01 μmoles min−1 mL−1).

Keywords

Newer insecticides, acetylcholinesterase, carboxylesterase, glutathione-S-transferase, Spodoptera litura, groundnut