1Crop Production Division, ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, 700 120, West Bengal, India
2Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
3Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
4ICAR-Vivekanand Parvatiya Krishi Anusandhan Sansthan, Almora, 263 601, Uttarkhand, India
*Corresponding author E-mail: viswa_iari@hotmail.com
Online published on 11 July, 2018.
A simple laboratory method for quantification of indole-3-acetic acid in rice genotypes involving of extraction, clean up and estimation by HPLC, using fluorescence (FLD) and variable wavelength (VWD) detectors in short run of 15 min is reported. Method performance was established by analyzing fortified rice samples through intra-and inter-d precision. It was validated through calibration curve, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. Retention time (RT) varied from 8.04–8.17min. Among the two detectors, FLD showed higher sensitivity with average recoveries from 80 to 95 per cent with acceptable precision.
Indole-3-acetic acid, HPLC, method, rice
