1Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore641 003, Tamil Nadu, India
2Pesticide Toxicology Laboratory Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India
*Corresponding author E-mail: sivam25@gmail.com
Online published on 23 March, 2021.
A gas chromatography-mass spectrometry method was developed and validated for the determination of indoxacarb residues in cabbage, chilli and tomato, and soil, as per SANTE guidelines. The validation parameters included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, precision, matrix effect, ion ratio and retention time. Sample preparation involved the extraction of residues from matrices using acetonitrile followed by dispersive solid phase extraction clean up. The LOD and LOQ were 0.003 and 0.01 μg g−1, respectively. Recoveries ranged from 84.03 to 103.80 per cent and 82.51 to 90.89 per cent from vegetables and soil with RSD of 3.12 to 6.36 and 1.75 to 3.79 per cent respectively. The matrix effects were observed between -4.68 to 8.12 per cent and the analytical method was reproducible (HorRat < 0.5) at three different studied concentrations. The validated analytical method could be an alternate method to existing LC based method for determination of indoxacarb residues in vegetables.
Indoxacarb, GC-EI-MS, Vegetables, Soil, Hor Rat
