Department of Entomology, Punjab Agricultural University, Ludhiana-141 004, Punjab, India
*Present address for correspondence: Assistant Entomologist, Punjab Agricultural University, Regional Fruit Research Station, Abohar - 152 116, Punjab (India)
Online published on 3 November, 2011.
A HPLC procedure has been described for the analysis of diflubenzuron in mushroom. The residues from fortified mushroom samples were extracted with methylene chloride and cleanup was carried out in miniature silica gel columns and Florisil Sep-Pak cartridges. Methylene chloride and acetonitrile were used as eluting solvents. The eesidues were eissolved in acetonitrile and analysed by HPLC (acetonitrile: water, 60: 40, v/v). Recoveries of diflubenzuron from Silica gel miniature columns and Florisil Sep-Pak cartridges while using 20 ml methylene chloride as eluting solvent at the fortification level of lmg kg−1 were 90.68 ± 6.96% and 88.13 ± 6.46%, respectively. The recovery from Silica gel miniature column was maximum (94.95 ± 4.66%) at fortification level of 0.1 mg kg−1 when methylene chloride was used as eluting solvent. Acetonitrile as eluting solvent gave adequate recoveries with Silica gel miniature columns and Florisil Sep-Pak cartridges at fortification levels of 0.1 and 1.0 mg kg−1, respectively.
Diflubenzuron, analysis, HPLC, mushroom, residues
