1University of Chinese Academy of Sciences, Beijing-100 049, China
2Da'an Sodic Land Experiment Station, Da'an, Jilin-131 317, China
3Disease Resistant Crop Research and Development Unit, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba-305 8602, Japan
4Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, Heilongjiang-150 081, China
North-east Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin-130 102, China
*E-Mail: cjjiang@affrc.go.jp
Online published on 27 March, 2014.
Proline accumulation is a common physiological response to various abiotic stresses in plants. In this study, we show that rice seedlings accumulate proline to different levels in response to different saline-alkaline stress factors including osmotic, saline and alkaline stresses. Expression analysis of genes for proline biosynthesis (OsP5CS1, OsP5CS2 and OsP5CR) and catabolism (OsPDH1 and OsP5CDH) revealed up-regulation of the proline synthesis genes under osmotic and alkaline stresses. In comparison, under the salt stress, a decrease in catabolic enzyme gene expressions was observed. Our results suggest that rice seedlings employ different gene regulation in proline accumulation in response to different saline-alkaline stress factors.
Proline accumulation, saline-alkaline stress, stress response, transcriptional regulation