Comparison of two methods for DNA extraction from guava (Psidium guajava)

Psidium guajava is well known tropical tree which is abundantly grown for fruit. This fruit plant is consumed as food and folk medicine in sub-tropical areas worldwide due to its pharmacological activities. Guava finds a very important place in medical systems almost worldwide. This study was conducted in 2022 at the genetic lab, University of Limpopo to compare two different extraction methods for DNA from guava. The DNA from the guava was extracted using Chelex “method 1” (overnight incubation at 56°C) and “method 2” (Ten-minute incubation at 95°C) from the fresh leaves of the guava. The quantitative and qualitative parameters were measured using a spectro photometer. PCR reactions with primers for 28S were used on all samples to confirm and evaluate the extracted DNA. The results showed that the highest quality of extracted DNA was in “method 2” (1.68–1.82). The quantitative and qualitative tests for PCR reaction showed that the DNA extracted using “method 2” had a better quality of DNA than “method 1”. In conclusion, both methods worked, but method two showed better results.