Grapes recovery from grapevine fan leaf virus by chemotherapy using salicylic acid

This paper presents the results of obtaining improved regenerants of Vitis vinifera L. grapes from fan leaf virus (FLV) using biotechnological methods. The parent plants were tested for viruses using the multiplex polymerase chain reaction (PCR) method and the Taq PCR kit (New England Biolabs). The RT-PCR results showed the presence of FLV in Vitis vinifera L. variety Aisulu and its absence in the Medeo and Alma-Ata varieties. The recovery method is based on the use of apical meristem culture with a relative explant size of 0.1-0.2 mm and 0.8-1.0 mm in combination with chemotherapy, which was carried out on Murashige-Skoog medium with the addition of 30 mg/L salicylic acid for three passages in vitro. The work shows that the combination of apical meristem and chemotherapy provides high recovery of grape plants in vitro from FLV. The study was conducted in 2022-23 in the Plant Bioengineering Laboratory of the M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry.