1Department of Biotechnology, Bharathidasan University College, Perambalur-621 212
2Department of Biotechnology, St. Joseph College, Trichy –620 002
3P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamilnadu, India.
*Corresponding Author: chola_rajan2000@yahoo.com
Online published on 21 February, 2013.
The role of aluminium and phosphorus on the secretion of acid phosphatase in the callus culture of Brassia juncea was studied. Callus culture derived from leaves of mustard (Brassica juncea) plants. Proliferated calluses were subjected to P-deficient as well as P-sufficient conditions in the presence and absence of aluminium. The 10 days old calluses were harvested and so the culture medium in which they were cultured, for analysis of acid phosphatase, protein and amino acid content.
Tissue culture, Brassica juncea, Callus
