Research Journal of Engineering and Technology
  • Year: 2013
  • Volume: 4
  • Issue: 4

A complete study on biodegradation of Reactive Red C2G29 using newly isolated Acinetobacter sp.

  • Author:
  • D. Suresh1, P. Sridhar1, D Joyce Hellen Sathya2, M. Anil Kumar2, M. Seenuvasan2, R. Dhandapani3,
  • Total Page Count: 5
  • Page Number: 246 to 250

1Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode

2Department of Biotechnology, Madha Engineering College, Kunrathur, Chennai

3Department of Microbiology, Periyar University, Salem

*Corresponding Author Email: danpani@gmail.com

Online published on 31 October, 2013.

Abstract

A bacterium designated as Acinetobacter sp., isolated from domestic sewage soil identified by taxonomic studies by 16S rDNA sequential analysis was found to decolorize azo dye with the decolorization efficiency varying from 80–96%. This strain decolorized 96.2% a deep red sulphonated azo dye Reactive RedC2G29 (200 mg/L) with 1.76 g/L dry cells of specific decolorization rate in static condition at optimum pH 5.0 and temperature 30°C. The degradation efficiency of this strain was within 48 h. Phytotoxicity studies revealed less toxic nature of decolorized products as compared to original dye. HPLC, GC-MS and LC-MS analysis revealed the production of metabolites as final product by degradation of the dye.

Keywords

Reactive Red C2G29, Degradation, Acinetobacter sp