Gold Nanoparticle for Protein Delivery

New biologic drugs such as proteins and nucleic acids require novel delivery technologies that will minimize side effects and lead to better patient compliance. Hence, various research using Nanoparticle as carrier has been done for the delivery of gene, drug, photo acoustic imaging and LASER induced photo thermal therapy. In our research, we have studied the delivery of an enzyme using nanoparticles as a carrier in microorganism. The enzyme which is capable of cleaving the peptides is made inactive by inhibitors present inside the cell. But we have studied that the enzyme can be made resistive by coating with nanoparticles. The action of this enzyme has been analyzed on a specific enzyme. It has been selected as target because one enzyme can't cleave other enzyme in active form but its peptides can be cleaved inside the cell alone. In this study, we have used complex system of an enzyme and nanoparticles to cleave the enzyme inside the cell which shows the successful delivery of the protein. In addition, by combining with nanoparticles, the intracellular stability of the enzyme can be maintained. These results suggest that a protein or drug can be conjugated with nanoparticles against a specific disease.