Method Development and Validation of Canagliflozin by using RP-HPLC in Pure and Tablet Dosage Form

A new method was established for simultaneous estimation of Canagliflozin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Canagliflozin by using INERTSIL column, C18 (150x4.6)5ìm column, flow rate was 1ml/min, mobile phase ratio was Water: Acetonitrile (70:30), detection wavelength was 264nm. The instrument used was Hitachi HPLC Auto Sampler, Separation module 1575. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study for Canagliflozin was found in concentration range of 1ìg-5ìg and 100ìg-500ìg and correlation coefficient (r2) was found to be 0.999 and 0.999, !mean recovery was found to be 100! and 100.5!, !RSD for repeatability was 0.2 and 0.4, !RSD for intermediate precision was 0.5 and 0.1 respectively.