Studies on the Compound Separation and Its Antifungal Potentiality of Fungi Isolated From Nagapattinam District, East Coast of India

Altogether 37 species belonged to 10 genera were recorded. A preliminary screening of all the species isolated from soils were used for antifungal (antagonistic) activity against Fusarium semitectum, a known soil borne fungal pathogen. Among the species tested, Trichoderma koeningii showed promising activity, inhibited the pathogenic fungus for the maximum both in dual culture and in food poisoning technique. When HPLC fractionation of the mycelial extract of T. koeningii grown in PDA broth yielded three fractions. They were named as peak 1, peak 2 and peak 3.Among the three fractions, peak 2 alone showed the antifungal activity with the inhibition zone of 13 mm diameter against Fusarium semitectum. The functional groups present in peak 1 and 3 are almost same, but in the peak 2 some extra functional groups are present. These groups may be responsible for the antifungal activity of this fraction.