1Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Secunderabad
2Sir C.R. Reddy College of Pharmaceutical Sciences, Eluru
*Corresponding Author: B. Siddartha, Assistant Professor, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad-500014. Andhra Pradesh, India. siddarthabethi@rediffmail.com
Online published on 3 February, 2014.
A simple, precise and accurate UV Spectrophotometric method has been developed for estimation of Pantoprazole in bulk and tablet dosage form. In this method Pantoprazole shows λmax at 295nm using 0.1N NaOH as a solvent and calibration graphs were plotted over the concentrations ranging from 1 to 20μg/ml of Pantoprazole with correlation coefficient 0.999. The proposed method was validated as per ICH Q2 (R1) guidelines for precision, linearity, accuracy and recovery. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.083μg/ml and 0.250μg/ml respectively by simple UV spectroscopy.
Pantoprazole, UV-Spectroscopy, Validation, NaOH, ICH guidelines
