Research Journal of Pharmaceutical Dosage Forms and Technology
  • Year: 2014
  • Volume: 6
  • Issue: 1

Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum

  • Author:
  • Subhadeep Ganguly1, Kunja Bihari Satapathy1,, Ajit Kumar Banik2
  • Total Page Count: 6
  • Page Number: 1 to 6

1Post-Graduate, Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha

2Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal, India

*Corresponding Author E-mail: res_biol@rediffmail.com

**Address for correspondence: Dr. Kunja Bihari Satapathy, Reader, Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha

Online published on 29 May, 2014.

Abstract

Corynebacterium glutamicum X1, a L-glutamic acid producing wild strain which accumulated mild amount of L-methionine (only 0.6 mg/ml) in the broth, was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogueresistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg/ml Lmethionine in the fermentation broth.

Keywords

Corynebacterium glutamicum, L-glutamic acid, L-methionine, analogue resistant