1CMJ University, Meghalaya
2Noida International University, Greater Noida
3Delhi University, New Delhi
4Head of Microbiology Lab., Dabur India Ltd., Ghaziabad
*Corresponding Author E-mail: manojkr11@rediffmail.com
**Address for correspondence: Manoj K. Sharma, Head of Microbiology Lab., Dabur India Ltd. Ghaziabad
Online published on 29 May, 2014.
In this study the production of cellulases from Aspergillus niger by solid state fermentation was performed using bio wastes viz; sugarcane bagasse and wheat bran. One factor at a time approach was used to optimize the variables such as pH, substrate ratio and temperature. A maximum of 988.2 IU/gm activity of enzyme is observed at substrates ratio of 2:1 (sugarcane bagasse: wheat bran) at 30°C and pH 5 after 5 days incubation. The high cost of production of Cellulotic enzymes has hindered the industrial application of cellulose bioconversion. Hence, this study showed an effective utilization of sugarcane bagasse for the production of cellulotic enzymes which could be effectively used in degradation of cellulose into smaller glucose units in a cost effective manner.
Sugarcane bagasse, Wheat bran, Aspergillus niger, cellulase, cellulose