Department of Botany, The Institute of Science, Fort, Mumbai400 032
*Corresponding Author E-mail: draparnasaraf@yahoo.co.in
Online published on 7 July, 2018.
Plants exhibits array of secondary metabolites which play a significant role in therapeutic activity of herbal drugs. Development of chemical fingerprints using High performance thinlayerchromatography (HPTLC) is an effective tool for linking the chemical constituents’ profile of the plant with botanical identity for estimation of chemical and bio chemical markers. Derris heyneana (Wight and Arn) Benth. has been attributed with antibacterial and antifungal activity.
To develop HPTLC fingerprint profile of various secondary metabolites from leaves of Derris heyneana (Wight and Arn) Benth.
The study involves the preliminary screening and extraction of various secondary metabolites from leaves of D. heyneana. The chemical fingerprint profile of individual secondary metabolite was established by HPTLC.
The HPTLC chromatogram of leaf extracts showed the presence of 11 Flavanoids, 7 Alkaloids, 8 Glycosides, 7 Phenols, 8 Saponins, 7 Steroids, 9 Sterols, 7 Tannins and 9 Terpenoids.
The chromatographic data generated and the HPTLC fingerprint developed may serve as a tool for standardization. Secondary metabolites can serve as drug prototype molecules known as “Lead Compounds”. The biochemical and physiological mechanisms can be understood using secondary metabolites as pharmacological probes.
Derris heyneana (Wight and Arn) Benth. Secondary metabolites, HPTLC
