Isolation, characterization of phytoconstituents from petroleum ether extract of Momordica balsamina fruits and its quantitative analysis by HPTLC

The purpose of the study is to isolate and characterize the chemical constituents from fruits of Momordica balsamina. The principal theme of the study is to develop applied chromatographic techniques for the separation, isolation and detection of the compounds. The petroleum ether extract of fruits of Momordica balsamina was saponified and unsaponifiable matter was subjected to column chromatography andelution of column was carried out by Petroleum ether (100%) with increasing concentration of ethyl acetate for the separation of phytoconstituents. The isolated compounds were characterized and analyzed by physical characteristics, IR, NMR and Mass spectroscopy. Four known compounds lupeol, ursolic acid, β-amyrin and β-sitosterol were isolated from fruits of Momordica balsamina. The isolated compounds were quantitatively analyzed by HPTLC. The concentration of lupeol, ursolic acid and β-amyrin was found to be 1.15%, 0.6% and 2.8%. From the present study, it is concluded that the plant consists of phytosterols and triterpene.