Phytochemical screening, total phenolic content and cytotoxic activity of seed, leaves, and pulp from Syzygium cumini against breast cancer cell culture 4T1

The exploration of plants as a source of medicinal raw materials has increased since the last few decades, both for infectious, degenerative, and malignant diseases. One of the medicinal plants currently being developed for cancer is Syzygium cumini. Powder of Syzygium cumini was extracted using 96% ethanol. Each extract was determined for its phytochemical screening, total phenolic content, and cytotoxic activity against 4T1 cell culture. Total phenolic content was determined by the Folin-Ciocalteu method and UV-VIS spectrophotometer. Cytotoxic test was performed using MTT assay with parameter IC₅₀ value. The results showed that the total phenolic content of 96% ethanol extract of Syzygium cumini seed, leaf, and pulp was 503.01±9.21; 329.60± 20.37; 24.09 ±0.57mg GAE/g. Syzygium cumini seed, leaf, and pulp extracts exhibited cytotoxic activity with IC₅₀ 613.92±40.49; 660.18±15.02; 732.68±69.41µg/ml. Syzygium cumini seed extract has the highest total phenolic content and the most potent cytotoxic activity.