Testing of antifungal activity of herbal cream made with natural herbal oils (Origanum vulgare, Melaleuca alternifolia, Anethum graveolans)

Natural herb oils of Origanum vulgare, Melaleuca alternifolia and Anethum graveolans were used to formulate a o/w emulsion type cream. Firstly, the authenticity of the cream was determined using reference standards such as thymol, carvone and cineole and then different phytochemical tests were carried out using standard procedures. The formulation of the cream was designed using Minitab software. The prepared cream was then subjected to antifungal assay. The synergistic effect of three herb oils were also studied and discussed.