Preliminary phytochemistry and HPTLC fingerprint profile of leaf extract of Latakaranj: A pharmaceutically significant plant

The prickly shrub Caesalpinia bonducella, also referred to as Latakaranj, is a plant part of the Caesalpiniaceae family and is used worldwide to treat a variety of conditions, including hypotensive, antihypertensive, anxiolytic, anticonvulsant, anti-inflammatory, analgesic, and antipyretic effects. The current study focuses on the fingerprinting profile of C. bonduc leaves using High Performance Thin Layer Chromatography and phytochemical screening. To identify different chemical elements such as alkaloids, glycosides, flavonoids, tannin and phenolic compounds, carbohydrates, fixed oil, steroid and terpenoids, proteins, amino acids, etc., a comparative phytochemical screening was conducted after hydro-methanolic extract was obtained. The mobile phase used for the HPTLC fingerprinting was n-hexane: Ethyl acetate (6:4% v/v). Several peaks with Rf values between 0.15 and 0.86 were found.