Research Journal of Pharmacognosy and Phytochemistry

Environmental stresses, including drought, salinity, and temperature fluctuations, are major challenges for plant survival. This study investigates Glyoxalase I (Gly I) in selected species of the Linderniaceae family, exploring its role in detoxifying methylglyoxal (MG), a cytotoxic byproduct under stress conditions. Using PCR and gel electrophoresis, we amplified partial sequences of the Glyoxalase I gene in Torenia crustacea (L) Cham. & Schltdl., Bonnaya ciliata (Colsm.) Spreng., Lindernia hyssopioides (L.) Haines. Optimal DNA and primer concentrations, along with annealing temperatures, were established, enabling precise amplification. Our findings suggest Glyoxalase I as a molecular marker for drought resilience, with broader implications for enhancing crop stress tolerance through genetic engineering. Future research should examine expression patterns across varied stress conditions to validate Glyoxalase I’s potential in crop improvement.