Detection and Estimation of Quercetin and Gallic Acid in Marketed Poly Herbal Formulation by HPTLC Method

A High-Performance Thin Layer chromatographic (HPTLC) method was developed for the qualitative and quantitative estimation of Quercetin and Gallic acid in the marketed poly herbal formulation. The method was developed using pre-coated HPTLC silica gel 60 F₂₅₄ as stationary phase and Toluene: ethyl acetate: formic acid: methanol (3:6:1.6:0.4, v/v/v/v) as mobile phase. Detection and quantification were performed at 254nm. The R_f values of Quercetin and Gallic acid was found to be 0.85 and 0.75 respectively. Linearity was observed in the concentration range of 0.5 to 2.5μg/spot. The accuracy of the method was confirmed by conducting recovery studies. The average recoveries of both the standards were close to 100% which indicates the accurateness of the method. Quantification of Quercetin and Gallic acid was found to be achieved by HPTLC fingerprinting.