1Modern College of Pharmacy, Nigdi, Pune
2R.C. Patel College of Pharmacy, Shirpur, Maharastra. India
3Amrutvahini College of Pharmacy, Tal-Sangamner, Dist-Ahmednagar, Maharastra, India
*Corresponding Author: Kalyani Abhimanyu Kedar Department of Pharmacognosy, Progressive Education Society's Modern College of Pharmacy, Sector -21, Yamunanagar Nigdi, Pune-411044, (MS) Phone: 09764012744 (M) E Mail: kk_pharma20@rediffmail.com
Online published on 21 March, 2013.
The present investigation evaluates the antioxidant activity of 7-methyl-1-phenyloctan-1-one of Helicanthus elasticus Linn. Stem (Loranthaceae) in various in vitro models. The n-hexane extract of Helicanthus elasticus Linn. (Loranthaceae) parasitic on Memecylon umbellatum Burm.f. (Melastomataceae) was subjected to column chromatography. The isolated compound was characterized by UV, IR, 1H NMR and Mass data. On comparing datas, it was confirmed that compound isolated from n-Hexane extract of Helicanthus elasticus was 7-methyl-1-phenyloctan-1-one. This is the first report stating the presence of such aromatic ketone from Helicanthus elasticus. The DPPH radical scavenging, nitric oxide scavenging and antilipid peroxidation activity of acetophenone derivative have IC50 value 10.23 μg/ml, 9.73 μg/ml, 16.45 μg/ml respectively. Thus results indicate that 7-methyl-1-phenyloctan-1-one showed potent antioxidant activity compare with standards, ascorbic acid and BHA.
The in vitro studies clearly indicate that the acetophenone derivative of Helicanthus elasticus Linn. Stem (Loranthaceae) has significant antioxidant activity.
Helicanthus elasticus, antioxidant activity, DPPH method, anti-lipid peroxidation
