1Department of Chemistry, College of Pharmacy, IFTM, Moradabad, (U.P.), India
2School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur, (Rajasthan), India-302025
*Corresponding Author: Vikas Gupta, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur (Raj.)-302025 E-mail: vikas.gupta1911@gmail.com, vikas_gupta1911@rediffmail.com Mob. +91-9252100551
Online published on 20 March, 2013.
This paper deals with the detailed phytochemical evaluation of the stem of Eclipta alba (Bhringaraja) family: (Compositae). The preliminary phytochemical screening shows that different extracts of plant have different constituents like carbohydrate, alkaloids, phenolics, steroids, glycosides, fat and oils. TLC of the hexane extract after column extraction was shown Rf value 0.78 in Petroleum ether: Hexane: Chloroform: Methanol, used as mobile phase in 2:4:2:1 ratio and TLC of pet. ether extract also shown Rf value is 0.72 in Petroleum ether: Hexane: Chloroform, used as mobile phase in 4:3:1 ratio after column extraction. This is evaluated by the instrumental analysis (UV, IR, and NMR) of hexane and pet. Ether extract. They shows consequently that pet. ether extract contains alkane (2940 Cm−1, δ 0.827), alkene (1630 Cm−1, δ 1.2531.351), monomer of aliphatic acid (1760 Cm−1, δ 2.169), aromatic amine (1570 Cm−1, δ 2.593) etc. and hexane extract contains alkane (2940 cm1, δ 0.801–0.873), alkene (1625 Cm−1, δ 0.988–1.075) aldehyde (1740 Cm1, δ 2.154 – 2.288) and aromatic alcohol (1110Cm−1, δ 5.324). These data suggests that the extracts (pet. ether and hexane) may have the glycoside, aliphatic acid, steroids, fats and oils. They are pharmacologically important.
Eclipta alba, flavanoids, glycosides, thin layer chromatography