Phytochemical screening and immunomodulatory activities of methanolic extract of Eclipta alba and Centella asiatica

An attempt has been made to assess the immunomodulatory activity of methanol extracts of whole plant of E. alba (1.6% wedelolactone) and C. asiatica (0.18% of asiaticoside) at five dose levels (dose-response relationship) ranging from 100 to 500mg/kg body wt. using carbon clearance, antibodytiter and cyclophosphamide immunosuppression parameters. In the case of E. alba, the phagocytic index and antibody titer increased significantly and the F ratios of the phagocytic indexand WBC count were also significant. Regression analysis showed linearity in patterns of the dose response relationship, greatest in the case of the phagocytic index, moderate in the WBC count andlowest in the antibody titer. For C. asiatica, significant increases in the phagocytic index and total WBC count were observed and the F ratio of the phagocytic index was also significant. Regressedvalues revealed maximum linearity in the case of the phagocytic index, moderate linearity in thetotal WBC count and lowest linearity in the antibody response.