1Institute of Zoology of the Ministry of Science and Education of the Republic of Azerbaijan
2Tokat Gaziosmasha University, Department of Basic Medical Sciences/Physiology, Turkey
3Scientific and Research Centre of Azerbaijan Medical University, Baku
4School of Education, General Education Program, ADA University, Azerbaijan
5Azerbaijan Medical University, Baku
6Institute of Radiation Problems of the Ministry of Science and Education of the Republic of Azerbaijan
The purpose of the work is to study the effect of a mixture of venom proteins and manganese nanoparticles on the composition of blood cells. Materials and methods. Experiments were carried out on 20 white outbred laboratory rats, weighing in the range of 200-220g, which were intraperitoneally injected with viper poison (Macrovipera lebetina obtusa) at a dose of 0.02mg/kg body weight and manganese nanoparticles. Research results and discussion. As a result, after the introduction of viper venom in combination with manganese nanoparticles, significant changes were observed in the blood of experimental animals. The content of leukocytes in the blood decreased by 12.1%, 19.5%, 26%, granulocytes, monocytes and neutrophils increased, respectively, by 8.6%, 3.8%, 3.9%. Analysis of daily blood showed that the content of leukocytes in the blood began to decrease, tending to intact values. The content of erythrocytes in the blood, on the contrary, during the entire study period increased by 2.5%, 4.2% and 8.5%, respectively. The content of red blood cells in the blood increased by 17.5% after 72 hours.
The results of laboratory studies showed that significant changes occur in the content of leukocytes and erythrocytes in the blood, which do not pose a threat to the animal’s body. Intraperitoneal administration of microdoses of viper venom mixed with manganese nanoparticles leads to quantitative changes in the composition of blood cells. Thus, intraperitoneal administration of a mixture of viper venom and manganese nanoparticles to white laboratory rats helps to normalize the composition of blood cells and stops the process of growth and reproduction of tumor cells and thereby eliminates the threat of tumor growth.
Viper Venom, Macrovipera Lebetina Obtusa, Leukocytes, Erythrocytes, Manganese Nanoparticles, Malignant Neoplasms
