Evaluating Effectiveness of Nigella sativa L Extract on Lipopolysaccharide-D-Galactosamine Induced Hepatic Toxicity in Rats

Nigella sativa is a promising natural remedy for liver problems because of its cytoprotective, hypolipidemic, and antioxidant properties. Here, we looked at the possible defense of Nigella sativa seed (NSS) against hepatotoxicity in rats caused by lipopolysaccharide and D-galactosamine. For 30 days, 36 adult Wistar albino rats were evenly and randomly split into six groups in order to achieve this goal. Lipopolysaccharide (30μg/kg b.wt., i.p.) and D-galactosamine (300mg/kg b.wt., i.p.) were given as supplements to the second group, whereas the control group received no therapy. Nigella sativa alcoholic extract (500 mg/kg b. wt orally) was added as a supplement to the third group. The fourth group was given D-GalN/LPS along with 500 mg/kg.B.wt. of Nigella sativa alcoholic extract. Group VI was given Thymoquinone (30 mg/kg b.wt. orally) in combination with D-GalN/LPS, whereas Group V was given Thymoquinone (40 mg/kg b.wt. orally) as a regular medication. NSS succeeded in boosting serum reduced glutathione level along with hemoglobin level. It reduced lipid peroxides in the serum along with serum bilirubin and ESR. NSS was successful in raising both the hemoglobin and serum reduced glutathione levels. Serum bilirubin, ESR, and lipid peroxides were all decreased. NSS's anti-apoptotic and antioxidant properties effectively guarded against the hepatotoxicity of DGalN/LPS. These results are extremely important since they highlight the use of NSS in our food sector and as a traditional medicine treatment to combat liver problems.