RMES’s College of Pharmacy, Old Jewargi Road, Balajinagar, Kalaburagi585102.
This study aimed to evaluate the effect of newspaper material extract (4 weeks) on adult zebrafish.
To determine the toxic effects of newspaper, extract on zebrafish liver and intestine.
48 adult zebrafish were procured from a local pet shop in Gulbarga and maintained in 8 round glass ponds. “OPTIMUM Highly Nutritious Food” was used as feed. Newspaper extracts (0.5%–10%) were prepared by adding weighed newspaper to 100mL water and squeezing out the extract.
Zebrafish were divided into 8 groups (6 per group); Group 1 was control, and Groups 2–8 received 0.5%–10% newspaper extract for 4 weeks, with incremental doses each week. Fish were fed twice daily, and ponds cleaned every evening. One fish per group was sacrificed at the end of the study, and liver with intestine was fixed in 10% formalin for histological examination.
Mean length of fishes after anesthesia was calculated.
Higher concentrations of newspaper extract increased risk of liver and intestinal damage.
Newspaper material, Liver toxicity, Zebrafish, Food packaging, Xylophagia
