1Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha-760002
2Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522001
3Department of Pharmacology, MKCG Medical College, Berhampur, Odisha-760004
*Corresponding Author: Ms. Saiprasanna Behera, Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha-760002 E-mail: behera.saiprasanna82@gmail.com; saiprasanna_behera@rediffmail.com Phone no – 09861252518
Online published on 21 February, 2013.
Myocardial infarction (MI) was produced in rats with 200 mg/kg of isoproterenol (ISO) administered subcutaneously (sc) twice at an interval of 24 h. Shift in antioxidant parameters, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), Troponin T, Catalase together with morphological and histopathological changes were investigated. Two hundred mg/kg ISO dose was selected for the present study as this dose offered significant alteration in biochemical parameters along with moderate necrosis in heart. Effect of pre-treatment of hydro-alcoholic extract of Ocimum canum (OC) at different doses (100, 200 and 400 mg/kg) was investigated against ISO (200 mg/kg) induced myocardial infarction in rats. Modulation of various biochemical parameters and membrane integrity was studied. OC at the dose of 200 and 400 mg/kg reduced significantly glutathione (GSH), superoxide dismutase (SOD) and LDH levels. It also inhibited the lipid peroxidation as observed by the reduced thiobarbituric acid reactive substances (TBARS) levels. In the present study OC at the dose of 400 mg/kg was found to demonstrate maximum cardio-protective effect. Above results were further confirmed by histopathological findings. Thus from the present study it is concluded that OC may be of therapeutic and prophylactic value in the treatment of MI
Isoproterenol, Myocardial infarction, Ocimum canum, Antioxidant enzymes
