Research Journal of Pharmacology and Pharmacodynamics

Analgesic and antiinflammatory activity of Amarwel extracts on experimentally induce pain and inflammation on animals

Petroleum ether extract, methanol extract, and aqueous extracts of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 and 300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acid-induced writhing response and formalin test. The anti-inflammatory activity of was determined using models likecarrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test, and leukocyte migration test using a single dose of 300 mg/kg orally.

The HPTLC analysis showed presence of quercetin in MECR and AECR (0.121 and 0.071 mg% respectively). Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/kg p.o.) significantly (P< 0.05) increased latency against thermal stimulus, decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema.

Results suggest significant analgesic and antiinflammatory effects produced by MECR and AECR.