1Asst Professor in Pharmacy, Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, India
2Principal and Head, Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore Tamilnadu, India
3Asst Professor in Pharmacy, Department of Pharmaceutical Chemistry, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, India
4Professor, Department of Pharmacognosy, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamilnadu, India
*Corresponding Author E-mail: mahi3kp@yahoo.co.in
**Address for correspondence: C. Maheswari, M.Pharm (Ph.D) Assistant Professor in Pharmacy. Department of Pharmacology, R.V.S. College of Pharmaceutical Sciences, Sulur, Coimbatore-641402
Online published on 28 August, 2014.
Aim of this study was to evaluate the in vivo anti-snake venom activity of leaves of Orthosiphon stamineus were studied against Cobra (NajaNaja) venom. The in vivo study was carried out by using Swiss albino mice in modifying the lethal effect of the test dose of the Cobra venom. In in vivo model the effectiveness of the extract was evaluated by oral administration of two different doses (200 and 400mg/kg) of the methanolic extract of the leaves of Orthosiphon stamineus 5 minutes prior to the injection of the venom and the percentage of mortality was observed. The extract markedly decreased the percentage of mortality in venom induced toxicity in mice at the dose of 400mg/kg b.w which indicates the significant anti-snake venom activity of the plant there by justifying its use in the indigenous system of medicine. The present study has confirmed the ethnomedical use of the plant for the treatment of snake bite.
Snake venom, NajaNaja, Orthosiphon stamineus, Leaves, Mice
