Preliminary Phytochemical Screening and In Vitro Antioxidant Properties of the Pleurotus ostreatus extract

The free radical amassed in the body due to oxidative stress, and plays the leading part in the inception of disease. Presently researchers are working on natural antioxidant, because it has minimum toxic effect compared to chemical substances. Pleurotus ostreatus extract were evaluated for phytochemical composition and to assess in vitro antioxidant activities. In the course of preliminary phytochemical analysis, the petroleum ether extract, ethyl acetate extracts and ethanol extracts of P. ostreatus was evaluated to determine the presence of secondary metabolites. Antioxidant activity of the ethanol extract of P. ostreatus was assessed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Super oxide free radical scavenging assay (SOD) and total polyphenol content. Preliminary phytochemical screening of ethanol extract revealed the presence of phenolic compounds, flavonoids, alkaloids and glycoside, while petroleum ether and ethyl acetate extracts were devoid from these phytoconstituent. The ethanol extract scavenged DPPH radical and superoxide radical with the IC₅₀ being 69.30 μg/ml and 107.05 μg/ml respectively. The ethanol extract of P. ostreatus revealed 83.47±1.53 mg/100 gm of total polyphenol content. The finding endorses that P. ostreatus acquire high antioxidant activities, and it may uses as natural antioxidant for the healing of various diseases.