Simultaneous Estimation of Metoprolol and Amlodipine from Tablet Dosage Form

Simple spectrophotometric methods have been developed for simultaneous estimation of Metoprolol (MET) and Amlodipine (AMD) in two component tablet formulation. The methods employed are Absorbance corrected for interference method and Area under curve method. For Absorbance corrected for interference method the working wavelengths selected are 224 nm for MET and 362.5 nm for AMD. Similarly for Area under curve method the working wavelength ranges selected are 234–242 nm (λ₁ – λ₂) for AMD and 218–228 nm (λ₃ – λ₄) for MET. For both the methods linearity was observed in the concentration range of 10–50 μg/ml for MET and 4–20 μg/ml for AMD. The recovery studies confirmed the accuracy of proposed method and the methods were validated as per ICH guidelines.