Estimation of Balsalazide in Bulk and in Formulation by UV-Visible Spectrophotometry

Three simple, precise and accurate UV-Visible spectrophotometric methods were developed for the estimation of Balsalazide in bulk drug and in pharmaceutical formulations. In method A, UV spectra of Balsalazide in double distilled water exhibits the absorption maxima at 358 nm. Method B is based on the formation of a dark red colored chromogen in alkaline medium at pH 12 exhibits λ max at 456 nm. Method C is the derivative spectroscopic method. The first derivative spectra were recorded between 200 and 500 nm at N=1 and the assay was carried out by measuring the absorbance at 383 nm. Beers law obeyed in the concentration range of 2–20 μg/ml for methods A and B and 5–25 μg/ml for method C. The accuracy of the method was determined by recovery studies. The method were validated statistically. The methods showed good reproducibility and recovery with % RSD less than 2. The methods were found to be simple, economical, accurate and reproducible and can be used for routine analysis of balsalazide in bulk drug and in pharmaceutical formulations.