Spectrophotometric Method for Simultaneous Estimation of Amlodipine Besylate and Bisoprolol Fumarate in Pharmaceutical Preparations

Simple spectrophotometric method has been developed for simultaneous estimation of amlodipine besylate and bisoprolol fumarate in combined dosage form. The method employed simultaneous equation method for analysis using 10% methanol as a solvent. The two wavelengths 222 nm and 365 nm were selected for estimation of bisoprolol fumarate and amlodipine besylate respectively. Linearity was observed in the concentration range of 5–100 μg/ml for both the drugs amlodipine besylate and bisoprolol fumarate. The recovery studies ascertained the accuracy of the proposed method and the results were validated as per ICH guidelines. The method can be employed for estimation of pharmaceutical formulations with no interference from any other excipients and diluents.