New validated RP-HPLC method for the determination of Vilazodone hydrochloride-A serotonergic Anti-depressant

A new simple liquid chromatographic method has been established for the determination of Vilazodone hydrochloride in pharmaceutical formulations. Vilazodone hydrochloride is a strong dopamine antagonist. Vilazodone hydrochloride was approved by the FDA for use in the United States to treat major depressive disorder in January 21, 2011. A mobile phase containing0.1M Ammonium formate: Methanol (20: 80, v/v) with flow rate 0.7 mL/min was used for the present chromatographic study(UV detection 241 nm). Linearity was followed over the concentration range 0.1–120 μg/mL. Forced degradation studies were performed by exposing the drug Vilazodone hydrochloride to alkaline, acidic, and oxidation stress degradations. The method was validated as per ICH guidelines (ICH Guidelines 2005).