Simultaneous determination of Ketorolac tromethamine and Fluorometholone in Eye drops by spectrophotometry

Four simple spectrophotometric methods have been developed for the simultaneous determination of Fluorometholone and Ketorolac tromethamine in ophthalmic preparations. Simultaneous equation method, absorbance ratio method (Q-Analysis), first derivative method and multicomponent mode of analytical techniques were selected for the determination of Fluorometholone and Ketorolac tromethamine in phosphate buffer (pH 2.0) solution. Fluorometholone and Ketorolac tromethamine has followed linearity over the concentration range 0.1–20 μg/mL and 1–20 μg/mL respectively. The methods were validated and applied for the simultaneous determination of Fluorometholone and Ketorolac tromethamine in the available pharmaceutical formulations i.e. eye drops.